Metabolic Profiling of Urinary Chiral Amino-Containing Biomarkers for Gastric Cancer Using a Sensitive Chiral Chlorine-Labeled Probe by HPLC-MS/MS.
- Rongrong Huang, Kexin Shen, Quan He, Yiqiu Hu, Cuirong Sun, Cheng Guo, Yuanjiang Pan.
-
Ion-Pairing Chromatography and Amine Derivatization Provide Complementary Approaches for the Targeted LC-MS Analysis of the Polar Metabolome. Grover, Karsten Siems, Francesca Cirulli, Alessandra Berry, Chiara Musillo, Ian D.
- Virag Sagi-Kiss, Yufeng Li, Matthew R.
- This article is cited by 93 publications. With the inclusion of antioxidant and reducing agents, tris(2-carboxyethyl)-phosphine (TCEP) and ascorbic acid, into both extraction solvents and reaction buffers, degradation was significantly decreased, allowing reproducible identification and quantification of amine compounds in large sample sets. The stability of derivatized amines was found to be variable with oxidatively labile derivatives rapidly degrading.
Further, with the use of accurate mass, charge state, and retention time, identification of unknown amines is facilitated. With the use of untargeted fragmentation scan functions, such as precursor ion scanning, the loss of the aminoquinoline tag (Amq) can be monitored to identify derivatized species and the use of targeted fragmentation scans, such as multiple reaction monitoring, can be exploited to quantitate amine-containing molecules. Upon collision induced dissociation (CID) in a quadrupole collision cell, all derivatized compounds lose the aminoquinoline tag. Complex mixtures of these amine derivatives can be fractionated and quantified using liquid chromatography–electrospray ionization-mass spectrometry (LC–ESI-MS). Primary and secondary amines, including amino acids, biogenic amines, hormones, neurotransmitters, and plant siderophores, are readily derivatized with 6-aminoquinolyl- N-hydroxysuccinimidyl carbamate using easily performed experimental methodology.